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SnCE1 locates to the endoplasmic reticulum with observed increase in mitochondrial fragmentation To confirm the N-terminus being important for subcellular localization of SnCE1 in the host cell, the SnCE1 acetylation state modulating its autoproteolytic processing and to uncover the cellular processes regulated by SnCE1 in the host cell, we next performed subcellular fractionations and microscopy of human cells expressing SnCE1 and variants thereof